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KMID : 0360220130540030496
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Journal of the Korean Ophthalmological Society
2013 Volume.54 No. 3 p.496 ~ p.501
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Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells
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Lee Keun-Woo
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC). Methods: Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 μm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay. Results: DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides. Conclusions: DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.
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KEYWORD
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Antioxidant, Dipyridamole, Reactive oxygen species, Trabecular meshwork cells
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